Capillary electrophoresis with a chemiluminescence detector using the two reactions of luminol and peroxyoxalate

摘要

We investigated capillary electrophoresis (CE) with a chemiluminescence (CL) detector using both CL systems of luminol and peroxyoxalate. Flow- and batch-type CL detection cells were used to analyze model samples, such as isoluminol isothiocyanate (ILITC), fluorescein isothiocyanate (FITC), and proteins labeled with these reagents. CE with the flow-type cell allowed analysis of hemoglobin, FITC, and FITC-labeled hemoglobin. Hemoglobin and FITC were detected with the luminol CL system and peroxyoxalate CL system, respectively, in the CE apparatus. On the other hand, FITC-labeled hemoglobin showed elevated CL with both luminol and peroxyoxalate CL systems. The detection limit of FITC-labeled hemoglobin was ca. 0.6 μM. The CE apparatus with batch-type cell was used to analyze ILITC, FITC, and ILITC-, FITC-, and ILITC/FITC-labeled human serum albumin (HSA). ILITC and ILITC-labeled HSA as well as FITC and FITC-labeled HSA were detected with the luminol CL system and with the peroxyoxalate system, respectively. ILITC/FITC-labeled HSA showed elevated CL with both CL systems.