Countercurrent chromatographic separation of hippuric acid and related compounds using cross-axis coil planet centrifuge with eccentric coil assemblies

摘要

Countercurrent chromatographic separation of hippric acid (HA) and related compounds was performed using the cross-axis coil planet centrifuge equipped with a pair of eccentric coil assemblies mounted in an off-center position. Partition coeffi- cients of HA and a set of related compounds were determined on a polar two-phase solvent system composed of methyl t-butyl ether, I-butanol, acetonitrile and aqueous 0.1% trifluoroacetic acid at various volume ratios. The optimal volume ratio of 1 : 0 : 0 : 1 was successfully used to resolve p-amino HA, HA, p-methyl HA, and (:f:)-mandelic acid by the lower aqueous phase mobile, and benzoic acid, p-methyl HA and HA by the upper organic phase mobile.