A Rapid Simple Approach to Screen Componentsin Radix Salviae Miltiorrhizae Using RapidResolution Liquid Chromatography Coupled withDiode Array Detection and Electrospray IonizationTime-of-Flight Mass Spectrometry

摘要

An improved method based on rapid resolution liquid chromatography (RRLC) coupled with diode array detection (DAD) and electrospray ionization time-of-flight mass spectrometry (TOF/MS) has been developed for quantification of seven major components and identification of most components in extracts of Radix Salvia Miltiorrhizae (RSM). RRLC analysis was performed with a C_(18) column by gradient elution using acetonitrile and 0.4% formic acid (v/v) in water as the mobile phase; the detection wavelength was set at 280 nm. Seven major components in RSM were successfully separated. This quantitative method was fully validated with respect to the following performance criteria: linearity, precision, repeatability, stability, accuracy, limit of detection (LOD) and quantification (LOQ). The UV spectra, acquired by DAD, were used to distinguish the two groups of components roughly, water-soluble phenolic and nonpo-lar diterpenoid compounds. A formula database of known compounds in RSM was established, against which most of the reported components in the herbal extract were identified effectively, based on the extract masses acquired byTOF/MS. This qualitative and quantitative method was successfully used to analyze the components in 10 batches of RSM samples collected from different regions in China. This improved quality evaluation method consisted of RRLC assay of seven major components and unambiguous identification of forty components, is suitable for routine quantification and comprehensive quality control of RSM.