Amperometric detection of estradiol and its metabolites in menstrual women and the urine of pregnant rabbits

摘要

A liquid chromatography method with electrochemical detection has been developed for the quantitative measurement for the five estrogen derivatives (estrone, estradiol, estriol, 2-methoxyestrone, and ethinylestradiol) in human and rabbit urine. The detection cell consisting of a dual thin-layer glassy carbon electrochemical signal was obtained with a supporting electrolyte containing 40% methanol-25% acetonitrile-35% phosphate buffer (pH 6.0) as the mobile phase. The method was applied for the determination of the four compounds in menstrual women and the urine of pregnant rabbits.