Evaluation of the bacterial anti-adhesive properties of polyacrylic acid, chitosan and heparin-modified medical grade Silicone rubber substrate

摘要

In this study, we aimed to develop polyelectrolyte multilayered films that could be absorbed onto a silicone rubber substrate; this composite material was then tested for its ability to inhibit bacterial adhesion. Polyacrylic acid (PAA) grafting onto the silicone rubber substrate was confirmed by attenuated total reflectance-infrared spectroscopy and the surface roughness of multilayered films was measured using an atomic force microscope and scanning electron microscopy. Immobilization of PAA, chitosan (CS) and heparin (HEP) films conferred antibacterial activity against Escherichia coli and Staphylococcus Aureus. The adhesion of both E. coli and S. aureus on PAA-CS-HEP multilayer film was reduced when compared to pristine, PAA, and PAA-CS films; this was due to effective complex formation between polyelectrolytes and cell adhesion proteins. Moreover, immobilization of HEP on the PAA-CS composite was efficiently reduced cell adhesion.