DETERMINATION AND ANALYSIS OF HUMAN SERUM ALPHA-1-ACID GLYCOPROTEIN BY LIQUID CHROMATOGRAPHIC METHODS

摘要

Human serum alpha-l-acid glycoprotein (orosomucoid, AGP) with particular interest as a tumor marker was measured, separated, purified and analyzed for its carbohydrate constituents. In order to demonstrate the changes in the concentration and composition of serum AGP induced by malignant diseases an improved method of sample preparation based on solvent extraction, ion exchange and reversed phase liquid chromatographic methods were applied. In preliminary studies significantly elevated serum AGP levels and increased fucose content in the carbohydrate moiety were found in cancer patients in comparison to the healthy individuals.