Propagation of a Field Isolate of Avian Pox Virus on Chorioallantoic Membrane of Developing Chicken Embryo and in BGM-70 Cell Line

摘要

A field isolate of Fowl pox virus (FPV) was grown successfully on chorioallantoic membrane (CAM) of developing chicken embryo and Baby Gravid Monkey Kidney cell line (BGM-70 cells), as evident by different degree of Cytopathic effects (CPE). Studies were conducted on the pathogenicity of these viruses in these different host systems which produced pock lesions in CAM infected with FPV with extensive congestion and foci of hemorrhages covering entire inoculated site. The cytopathic effects in infectedBGM-70 cell monolayers consisted of rounding and clumping of cells, vacuolation, macro and micro plaques and intracytoplasmic inclusion bodies. The cytopathic effects were seen in both unstained as well as MGG stained monolayers of BGM-70 cells