Expression of Bombyx mori Cecropin B in Silkworm Larvae using BmNPV/Bac-to-Bac Expression System

摘要

Antimicrobial peptides (AMPs) are a family of small molecular weight proteins produced by living organisms of all types, and have a broad range of antimicrobial activity against bacteria, viruses, and fungi. Thus it is important to efficiently express AMPs using Bombyx mori (Bm) baculovirus expression system. In the present study, we cloned Bm cecropin B (CecB2) and polyhedrin (polh) genes into the pFastBac DUAL vector. Recombinant baculovirus polyhedrin was generated by transfecting the recombinant plasmid into BmN cells and used to orally inoculate the silkworm larvae. We found that CecB2 was successfully expressed in the silkworm larvae and confirmed to have antibacterial activity to E. Coli, and ELISA quantative assay showed average concentration to 0.1151/4g/ml CecB2 was detected.