Studies of the Paclitaxel Delivery and Antimicrobial Activities Generated by MinD from Lactobacillus acidophilus VTCC-B-871

摘要

In this study, the minD gene from Lactobacillus acidophilus VTCC-B-871 was introduced into Escherichia coli BL21(DE3)plysS using pET 21(a+) vector. The overexpression under 0.5mM Isopropyl beta-D-1-thiogalactopyranoside within 5 hours caused filamentation and 24 hours caused minicells while in the glucose condition, the cells were filaments, interestingly. The minicells was packaged with paclitaxel at 10 mg/ml in 10 to 18 hours. After extraction of the packaged cells and high performance liquid chromatography performance, there was paclitaxel exisiting in the minicells. The study also tried to test antimicrobial activities and consequently, Pseudomonas aeruginosa ATCC 27853 was detected. The antimicrobial agent still showed the activities after treated with temperature at 100 degrees C, 90 degrees C, 80 degrees C, 70 degrees C, 60 degrees C in 15 minutes, but loss activity after protease K treatment at 10 mg/ml for 30 minutes. The characterization pointed the antimicrobial agent seemed a bacteriocin or colicin in Escherichia coli. As a result, MinD might affect on bacteriocin production in the host. The present work was the first report in characterizing the Lactobacillus MinD homolog that might be useful in antibiotic fields as well as drug delivery for paclitaxel.