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Isolation and Characterization of Anti Pathogenic Plant Growth Promoting Bacteria from Cotton Rhizosphere

机译:棉花根际抗病原菌生长细菌的分离与鉴定

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Sustainable farming systems strive to minimize the use of synthetic pesticides and to optimize the use of alternative management strategies to control soil borne pathogens. During the last decade numerous bio control experiments with bacteria have been reported. But when such bacterial formulation was tried on field, it was not able to give as nice response as its giving in lab. Therefore, it is necessary to develop efficient strains in field conditions. One possible approach is to explore soil microbial diversity for PGPR having combination of PGP activities and well adapted to particular soil environment. So keeping in view the above constrains, the present study was designed to screen certain rhizospheric bacterial isolates for their multiple plant growth promoting activities. In this research twelve bacterial isolates were successfully isolated from cotton rhizosphere which possess antagonistic activity against to main soil fungal pathogen of cotton as well as also shows plant growth promotingactivities. All the potent isolates were characterized phenotypically and biochemically. One of them were also characterized molecularly by 16s r RNA sequencing and found to be novel. The selected isolates are antagonistic to Rhizoctonia solani and Fusarium oxysporum and could be developed into a valuable crop management tool to reduce the deleterious impact of plant pathogen.
机译:可持续的耕作制度努力减少合成农药的使用,并优化使用替代管理策略来控制土壤传播的病原体。在过去的十年中,已经报道了许多关于细菌的生物防治实验。但是,当这种细菌制剂在野外尝试时,它不能像在实验室中那样给人很好的反应。因此,有必要在田间条件下开发有效的菌株。一种可行的方法是探索具有PGP活性并很好地适应特定土壤环境的PGPR的土壤微生物多样性。因此,鉴于上述限制,本研究旨在针对某些根际细菌分离株的多种植物生长促进活性进行筛选。在这项研究中,成功​​地从棉花根际分离了十二种细菌分离株,它们对棉花的主要土壤真菌病原体具有拮抗活性,并且还显示出促进植物生长的活性。在表型和生化上对所有有效分离株进行了表征。其中之一还通过16s r RNA测序进行了分子表征,被发现是新颖的。选定的分离株可拮抗茄根霉和尖孢镰刀菌,可被开发成一种有价值的作物管理工具,以减少植物病原体的有害影响。

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