Isolation and Genera Distribution of Hydrogen-oxidizing Bacteria in Loess Plateau Hippophae rhamnoides Rhizosphere

摘要

Rhizosphere of Hippophae rhamnoides was designated as research material. 39 bacterial strains were isolated by mineral salt agar (MSA) medium with a continuous H-2 supplying gas-cycling incubating system. Hydrogenase-positive strains were screened by both 2,3,5-triphenyltetrazolium chloride (TTC) and H-2 oxidizing capability tests. 6 strains with positive TTC result and strong H-2 oxidizing capability were determined to be hydrogen-oxidizing bacteria. The 6 strains were classified into genera of Bacillus sp, Aeromonas sp, Pseudomonas sp and Micrococcus sp by their cultural, morphology, physiological and biochemical characteristics. 16S rDNA sequence of strain FS2 has a homology of 99% with Bacillus sp and they are in the same branch of phylogenetic tree, indicating strain FS2 belongs to Bacillus sp.