Real Time RT RCR Detection of mRNA encoding Enterotoxin B Staphylococcus aureus in Synovial fluid of Patients withRheumatoid arthritis

摘要

Staphylococcal enterotoxins (superantigens) in Rheumatoid arthritis patients are considered. Nevertheless, there is unclear where is the origin of these superantigens in the SF of RA patients. This study aimed to assess mRNA encoding Staphylococcal enterotoxin B gene in Synovial fluid of the patients with RA. This experimental study, eighty Synovial fluids of patients with RA was assayed. The mRNA extraction carried out. Based on Staphylococcal enterotoxin B gene specific primers for RT Real Time PCRdesigned. Then, cDNA was synthesized and amplification by SYBR?Green Real Time RCR was down. The S. aureus strain ATCC 14458 as positive control was used. The results analyzed descriptively. The results indicated, using specific primers and probe for enterotoxin B could amplify the related cDNA gene. SYBR?Green Real Time RT RCR method has been outline that 38 cases (47.5%) were showed. In fact, the reverse transcriptase convert mRNA to cDNA for partially encoding staphylococcal enterotoxin B (superantigen) gene. In comparison positive and negative control, the results descriptively analyzed.The results showed, Real Time RT RCR were able to detect and characterized the mRNA producing enterotoxin B gene in Synovial fluid of 47.5% of the RA patients. Despite the finding may be discussed the direct role of enterotoxin B as a classic superantigen in the pathophysiology of RA disease. It may demonstrate the causative of disease and facilitate the rapid detection. However, more research is needed this finding could change the perspective approach treatment of the RA diseases.