Comparative Study of Dark Field Microscopical Examination, Microscopic Agglutination Test and Polymerase Chain Reaction in the Diagnosis of Bovine Leptospirosis

摘要

A total of 115 serum samples (33 from cattle and 82 from buffaloes) collected from different clinical cases (65 Nos) like abortion, repeat breeding, jaundice and haemorrhagic mastitis and also from apparently healthy bovines were subjected to Dark field microscopical examination. Microscopic agglutination test and Polymerase chain reaction to diagnose bovine leptospirosis. The present study showed high positivity in MAT (58.46 %) followed by DFM (38.46 %) and PCR (32.31 %). The study concluded to useMAT for serosurveillance study and MAT followed by PCR for the diagnosis of leptospirosis.