Comparison of HPTLC, HPLC, and HPCE for Fingerprinting of Pueraria Radix

摘要

To evaluate the reliability and applicability of chromatographic methods for fingerprinting, and for obtaining comprehensive information from the fingerprint, several chromatographic techniques including high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and high-performance capillary electrophoresis (HPCE) have been used to obtain fingerprints of Pueraria Radix for the first time. The results showed that the relative standard deviation (RSD) of R_F values, retention times, and peak-area percentages for samples of Pueraria lobata from different sources all perfectly satisfied the demands of the national standard, and HPTLC, HPLC, and HPCE have been successfully used for development of fingerprints of Pueraria Radix. When the chromatographic fingerprints established in this work were used to distinguish between authentic and fake Pueraria Radix. bv evaluation of similarity, different samples obtained from P. lobata and P- thomsonii were distinguished effectively. Although the three methods used for fingerprinting of Pueraria Radix were highly selective and reproducible, there were still significant reliability and applicability differences among the results obtained by use of these approaches, as is illustrated in this paper.