Synthesis of 4-diisopropylaminodiazabenzene-4'-isothiocyanate and its use in the derivatization and chromatographic separation of biogenic amines

摘要

The physicochemical properties of biogenic monoamines - their polarity, lability, and the trace amounts found in biological material - create great analytical difficulties in their identification and separation. The selective chromatographic separation of these amines is greatly facilitated by their derivatization. On the basis of theoretical assumptions 4-diisopropylaminodiazabenzene-4'-isothiocyanate (DIABITC) was synthesized and used to prepare the thiocarbamoyl derivatives of the amines. The colored derivatives were separated by TLC.