THE LEVEL OF A MRNA WITH SEQUENCE SIMILARITY TO THE OLD YELLOW ENZYME-NADPH DEHYDROGENASE INCREASES IN CHENOPODIUM RUBRUM CELLS IN RESPONSE TO CYTOKININ

摘要

Stationary-phase cells of Chenopodium rubrum were treated with t-zeatin riboside for 2 h, and the pattern of gene expression in both treated and non-treated cells was examined by differential display of mRNA. Based on this screen, we isolated a cDNA clone whose corresponding mRNA level increased upon cytokinin treatment. Northern blot analysis confirmed that expression of this mRNA was induced by t-zeatin riboside. The amino acid sequence deduced from the partial cDNA showed a high level of similarity to the corresponding amino acid sequence of the old yellow enzyme-NADPH dehydrogenases of fungi.