BETA-1,3-GLUCANASE MRNA IS LOCALLY, BUT NOT SYSTEMICALLY INDUCED IN NICOTIANA TABACUM L CV BEL W3 AFTER OZONE FUMIGATION

摘要

Ozone fumigation of tobacco caused a strong induction of basic beta-1,3-glucanase mRNA. In situ hybridization revealed a uniform labeling of the whole tissue section, except for the necrotic areas. Ozone-treated leaf parts showed an increased transcript accumulation, whereas no transcripts were detectable in nontreated leaf parts. Treatment of a single leaf increased beta-1,3-glucanase mRNA level only in this leaf and not in other leaves. Conversely, protection of a single leaf resulted in transcript accumulation only in the fumigated leaves. These results indicate that ozone-induced beta-1,3-glucanase mRNA accumulation is restricted to the ozone-treated area, and that ozone does not lead to systemic induction of this athogenesis-related> (PR) protein.