Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L - A Promising Biofuel Plant

摘要

An efficient in vitro plant regeneration from leaf-disc culture of Jatropha curcas L has been established. Adventitious shoot buds along with callus were induced from leaves of 2-year-old J. curcas plants cultured on Murashige and Skoog's (MS) medium supplemented with TDZ (2 mu M) BAP (2 mu M) and IBA (1 mu M), wherein 83.3% leaf explants responded. The multiplication of shoots was achieved from the adventitious shoot buds after transferring them to shoot induction medium. The highest number of shoots (9.7/explant) was achieved after 8 weeks of culture on MS medium containing 3 mu M of BAP. The well-developed shoots were rooted on MS medium supplemented with IBA (1.5 mu M) with the rooting frequency of 53.3%. Addition of phloroglucinol (200 mu M) to the medium enhanced the frequency of rooting to 76.7%. Regenerated plantlets were successfully transferred to field after initial acclimatization.