Selenomethionine Induced Changes on the Binding of Spermine with DNA: A Study by Fourier Transform Raman and Fourier Transform Infra Red Spectroscopy

摘要

In this research, FT Raman and FTIR spectroscopy had been used to extend our knowledge about spermine-DNA and selenomethionine-spermine-DNA interaction at different volume ratios. The analysis of FT Raman and FTTR data supported the existence of structural specificities in the interaction. From the observed results, the effect of spermine on DNA is reversed when selenomethionine is added with spermine-DNA complexation. For example, band at 1511 cm~-1 is assigned to an adenine carbon-carbon stretching vibration. This band shifts downward by 3 cm~-1 in the spectra of spermine-DNA of all the complexes studied. Similarly this band shifts upward by 2 cm~-1 in the spectra