Relationship between cell cycle and light-limited growth rate in oceanic Prochlorococcus (MIT9312) and Synechococcus (WH8103) (cyanobacteria)

摘要

The relationships between growth rate, cell-cycle parameters, and cell size were examined in two unicellular cyanobacteria representative of open-ocean environments: Prochlorococcus (strain MIT9312) and Synechococcus (strain WH8103). Chromosome replication time, C, was constrained to a fairly narrow range of values (similar to 4-6 h) in both species and did not appear to vary with growth rate. In contrast, the pre- and post-DNA replication periods, B and D, respectively, decreased with increasing growth rate from maxima of similar to 30 and 10-20 h to minima of similar to 4-6 and 2-3 h, respectively. The combined duration of the chromosome replication and postreplication periods (C+D), a quantity often used in the estimation of Prochlorococcus in situ growth rates, varied similar to 2.4-fold over the range of growth rates examined. This finding suggests that assumptions of invariant C+D may adversely influence Prochlorococcus growth rate estimates. In both strains, cell mass was the greatest in slowly growing cells and decreased 2- to 3-fold over the range of growth rates examined here. Estimated cell mass at the start of replication appeared to decrease with increasing growth rate, indicating that the initiation of chromosome replication in Prochlorococcus and Synechococcus is not a simple function of cell biomass, as suggested previously. Taken together, our results reflect a notable degree of similarity between oceanic Synechococcus and Prochlorococcus strains with respect to their growth-rate-specific cell-cycle characteristics.