Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress

摘要

The effects of zinc (Zn) on plant chlorophyll fluorescence were investigated in 10-day-old tomato (Solarium lycopersicum) seedlings subjected for 7 days to a series of zinc (10, 50,100 and 150 μM) applied via the nutrient solution. The chlorophyll fluorescence spectra of leaves were recorded in the spectral region 650-800 nm using the spectroscopic technique of ultraviolet light emitting diode induced fluorescence spectroscopy (UV-LED IFS). These spectra have been used to analyze the effect of several doses of zinc on the photosynthetic activities of tomato plants. The fluorescence intensity ratios (FIR) at the two maxima (F_(690)/F_(735)) of control as well as treated tomato plants were calculated by evaluating curve-fitted parameters using a Gaussian spectral function. The variable chlorophyll fluorescence decrease ratio (R_(Fd)) values were determined from the fluorescence induction kinetics curves recorded at 690 nm and 735 nm. In addition, Zn accumulation in plants, plant growth, photosynthetic pigments content and mal-ondialdehyde level (MDA, an index of lipid peroxidation) were also estimated. The results indicated that the plants treated with 10μM of zinc exhibited better growth, however, higher concentrations of zinc were harmful for plants. Excess Zn induced a decrease in the R_(Fd) values, which was associated with a strong decline of the total chlorophylls content and an increase of MDA level. The total chlorophylls content decline could also be followed via an increase of the chlorophyll fluorescence ratio F_(690)/F_(735).