Plum pox virus in Japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty.

摘要

A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; IC-RT-PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3' nc region. The analysis of the sequence showed the presence of the DAG motif at the 5' end of the capsid protein and the Rsa I and Alu I sites at the 3' end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5' N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3' terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.