Dynamics of polystyrene beads linking to DNA molecules under single optical tweezers: A numerical study using full normalized Langevin equation

摘要

The dynamics of a polystyrene bead linking to lambda-phage deoxyribonucleic acid (DNA) molecule under single optical tweezers using CW laser beam is analyzed by the full normalized Langevin equation with elastic and Brownian forces. The obtained results show that the stable time and stable position of polystyrene bead depend on its beginning position, laser beam waist, and laser beam power. The oscillation of polystyrene beads at stable position is observed with different parameters of optical tweezers. Finally, the conditions to stabilize the bead and to stretch the lambda-phage DNA by single laser beam optical tweezers are discussed.