首页> 外文期刊>Journal of Neuropathology and Experimental Neurology: Official Journal of the American Association of Neuropathologists, Inc >Distinct expression pattern of microtubule-associated protein-2 in human oligodendrogliomas and glial precursor cells.
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Distinct expression pattern of microtubule-associated protein-2 in human oligodendrogliomas and glial precursor cells.

机译:微管相关蛋白2在人少突胶质细胞瘤和神经胶质前体细胞中的不同表达模式。

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摘要

Microtubule-associated protein 2 (MAP2), a protein linked to the neuronal cytoskeleton in the mature central nervous system (CNS), has recently been identified in glial precursors indicating a potential role during glial development. In the present study, we systematically analyzed the expression of MAP2 in a series of 237 human neuroepithelial tumors including paraffin-embedded specimens and tumor tissue microarrays from oligodendrogliomas, mixed gliomas, astrocytomas, glioblastomas, ependymomas, as well as dysembryoplastic neuroepithelial tumors (DNT), and central neurocytomas. In addition, MAP2-immunoreactive precursor cells were studied in the developing human brain. Three monoclonal antibodies generated against MAP2A-B or MAP2A-D isoforms were used. Variable immunoreactivity for MAP2 could be observed in all gliomas with the exception of ependymomas. Oligodendrogliomas exhibited a consistently strong and distinct pattern of expression characterized by perinuclear cytoplasmic staining without significant process labeling. Tumor cells with immunoreactive bi- or multi-polar processes were mostly encountered in astroglial neoplasms, whereas the small cell component in neurocytomas and DNT was not labeled. These features render MAP2 immunoreactivity a helpful diagnostic tool for the distinction of oligodendrogliomas and other neuroepithelial neoplasms. RT-PCR, Western blot analysis, and in situ hybridization confirmed the expression of MAP2A-C (including the novel MAP2+ 13 transcript) in both oligodendrogliomas and astrocytomas. Double fluorescent laser scanning microscopy showed that GFAP and MAP2 labeled different tumor cell populations. In embryonic human brains, MAP2-immunoreactive glial precursor cells were identified within the subventricular or intermediate zones. These precursors exhibit morphology closely resembling the immunolabeled neoplastic cells observed in glial tumors. Our findings demonstrate MAP2 expression in astrocytic and oligodendroglial neoplasms. The distinct pattern of immunoreactivity in oligodendrogliomas may be useful as a diagnostic tool. Since MAP2 expression occurs transiently in migrating immature glial cells, our findings are in line with an assumed origin of diffuse gliomas from glial precursors.
机译:微管相关蛋白2(MAP2),一种与成熟的中枢神经系统(CNS)中的神经元细胞骨架相连的蛋白,最近已在神经胶质前体中被发现,表明在神经胶质发育过程中具有潜在作用。在本研究中,我们系统地分析了MAP2在一系列237种人类神经上皮肿瘤中的表达,这些肿瘤包括石蜡包埋的标本和来自少突胶质细胞瘤,混合神经胶质瘤,星形细胞瘤,胶质母细胞瘤,室管膜瘤以及胚性发育不良神经上皮性肿瘤(NT)的肿瘤组织芯片和中枢神经细胞瘤。另外,在发育中的人脑中研究了MAP2免疫反应性前体细胞。使用了针对MAP2A-B或MAP2A-D同工型的三种单克隆抗体。除脑室膜瘤外,在所有神经胶质瘤中均可观察到MAP2的可变免疫反应性。少突胶质细胞瘤表现出一致的强而独特的表达模式,其特征在于核周细胞质染色而没有明显的过程标记。具有免疫反应性双极或多极过程的肿瘤细胞在星形胶质细胞瘤中最常见,而神经细胞瘤和DNT中的小细胞成分未标记。这些特征使MAP2免疫反应性成为区分少突胶质细胞瘤和其他神经上皮肿瘤的有用诊断工具。 RT-PCR,蛋白质印迹分析和原位杂交证实了少突胶质细胞瘤和星形细胞瘤中MAP2A-C(包括新的MAP2 + 13转录物)的表达。双荧光激光扫描显微镜显示,GFAP和MAP2标记了不同的肿瘤细胞群。在胚胎的人脑中,在脑室下或中间区域内发现了MAP2免疫反应性神经胶质前体细胞。这些前体表现出与在神经胶质瘤中观察到的免疫标记肿瘤细胞非常相似的形态。我们的研究结果表明星形胶质细胞和少突胶质细胞肿瘤中的MAP2表达。少突胶质细胞瘤中免疫反应性的独特模式可能可用作诊断工具。由于MAP2表达在迁移的未成熟神经胶质细胞中短暂发生,因此我们的发现与假定的神经胶质瘤前体弥漫性神经胶质瘤起源一致。

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