Modified Strullu and Romand (MSR) medium devoid of sucrose promotes higher in vitro germination in Rhizophagus irregularis.

摘要

This study was conducted to evaluate the effect of sucrose on spore germination and germ-tube growth in Rhizophagus irregularis. Results showed that in vitro germination in MSR medium without sucrose was observed in 38 h after inoculation and recorded 90% germination whereas in MSR medium with sucrose germination was recorded after 60 h with 75% germination after 26 days. Germ tube length was significantly greater in MSR medium without sucrose. Average width of germ tube was higher in MSR medium without sucrose and was non-significant. The hyphae were longer and had fewer branches in MSR medium without sucrose, and were shorter with more branching in MSR medium with sucrose. The use of MSR medium minus sucrose is recommended to enable more rapid germination.