Large-Scale Protein Arrays Generated with Interferometric Lithography for Spatial Control of Cell-Material Interactions

摘要

Understanding cellular interactions with material surfaces at the micro- and nanometer scale is essential for the development of the next generation of biomaterials. Several techniques have been used to create micro- and nanopatterned surfaces as a means of studying cellular interactions with a surface. Herein, we report the novel use of interference lithography to create a large (4 cm~2) array of 33 nm deep channels in a gold surface, to expose an antireflective coating on a silicon wafer at the bottom of the gold channels. The fabricated pores had a diameter of 140-350 nm separated by an average pitch of 304-750 nm, depending on the fabrication conditions. The gold surface was treated with 2- (2- (2- (11-mercaptoundecyloxy)ethoxy)ethoxy)ethanol to create protein-resistant areas. Fibronectin was selectively adsorbed onto the exposed antireflective coating creating nanometer-scale cell adhesive domains. A murine osteoblast cell line (MC3T3-E1) was seeded onto the surfaces and was shown to attach to the fibronectin domains and spread across the material surface.