...
首页> 外文期刊>Journal of magnetic resonance >Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers
【24h】

Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers

机译:磷脂双层中的固态NMR顺磁弛豫增强浸没深度研究

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

A new approach for determining the membrane immersion depth of a spin-labeled probe has been developed using paramagnetic relaxation enhancement (PRE) in solid-state NMR spectroscopy. A DOXYL spin label was placed at different sites of 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC) phospholipid bilayers as paramagnetic moieties and the resulting enhancements of the longitudinal relaxation (T_1) times of ~(31)P nuclei on the surface of the bilayers were measured by a standard inversion recovery pulse sequence. The ~(31)P NMR spin-lattice relaxation times decrease steadily as the DOXYL spin label moves closer to the surface as well as the concentration of the spin-labeled lipids increase. The enhanced relaxation vs. the position and concentration of spin-labels indicate that PRE induced by the DOXYL spin label are significant to determine longer distances over the whole range of the membrane depths. When these data were combined with estimated correlation times τ_c, the r~(-6)-weighted, time-averaged distances between the spin-labels and the ~(31)P nuclei on the membrane surface were estimated. The application of using this solid-state NMR PRE approach coupled with site-directed spin labeling (SDSL) may be a powerful method for measuring membrane protein immersion depth.
机译:使用固态NMR光谱中的顺磁弛豫增强(PRE),开发了一种确定自旋标记探针的膜浸入深度的新方法。将DOXYL自旋标记物作为顺磁性部分放置在1-棕榈酰基-2-硬脂酰基-sn-甘油-3-磷酸胆碱(PSPC)磷脂双层的不同位置上,从而导致〜(31)的纵向弛豫(T_1)时间增强通过标准的反转恢复脉冲序列来测量双层表面上的P核。随着DOXYL自旋标记物移近表面,自旋标记脂质的浓度增加,〜(31)P NMR自旋晶格弛豫时间稳定减少。增强的自旋与自旋标记物的位置和浓度的关系表明,由DOXYL自旋标记物诱导的PRE对确定整个膜深度范围内的更长距离很重要。当将这些数据与估计的相关时间τ_c组合时,可以估计自旋标记与膜表面〜(31)P核之间的r〜(-6)加权时间平均距离。结合使用固态NMR PRE方法和定点自旋标记(SDSL)的应用可能是测量膜蛋白浸入深度的有效方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号