Surface Modification of GTA Crosslinked Collagen-based Composite Scaffolds with Low Temperature Plasma Technology

摘要

In this study for preparing the better performance scaffold materials for peripheral nerve repairing, the collagen-based composite scaffolds are crosslinked with glutaraldehyde and their structure and performance are investigated. The results of FTIR indicated that the collagen and chitosan are certainly crosslinked through GTA without any significant change in the chemical property. It was observed under a scanning electron microscope (SEM) that the crosslinked collagen-based composite scaffolds had a porous three-dimensional cross-linked structure. The experiments showed that the biostability of the scaffold is greatly enhanced, but the GTA crosslinking induces the potential cytotoxicity and poor hydrophilic nature. To overcome these disadvantages, the low temperature plasma technology is utilized to modify the surface of the cross-linked collagen-based composite scaffolds in this study. Measurements of water contact angle showed that hydrophilic nature of surface of the scaffolds was improved after low temperature plasma technology modification. The cell proliferation experiments revealed that the modified collagen-based composite scaffolds still kept their bioactivity and benefited the proliferation.