Water Glass Coating on a H Substrate to Form Si-OH Groups for Improving Cell Behaviors of Dental Implants

摘要

Some biomaterials bond with the surrounding living bone through a bone-like apatite layer which is formed on their surfaces in the human body. The existence of functional groups such as Si-OH and Ti-OH on the Ti surface accelerates the formation of a bone-like apatite layer in simulated body fluid (SBF) solution. In this study, Si-OH functional groups were formed on the Ti surface by water glass (WG, sodium silicate) coating and subsequent HCl-treatment. The thickness of the WG layers increased with an increasing concentration of WG solution and the number of WG coatings. The chemical composition of the WG coated layers changed from sodium silicate to pure silica by the HCl-treatment via ion-exchange. The content of hydroxyl groups on the HCl-treated samples was confirmed by TFAA treatment using XPS. The amount of hydroxyl groups on the Ti surface depended upon the vol% of WG and the number of WG coatings. The higher the concentration of WG and the greater the number of WG coatings, the more hydroxyl groups existed on the Ti surface. The cell (MSC) proliferation on the WG coated Ti was significantly improved compared to the non-WG coated sample due to the formation of hydroxyl groups on the WG coated Ti. The cell proliferation also depended on the WG coating thickness and the number of hydroxyl groups on the surface. That is, the increase in the number of hydroxyl groups on the Ti surface afforded by the WG coating resulted in better cell behavior. We conclude that Ti coated with WG can be employed for the early fixation of dental implants.