Characterization of Cellulase Gene (MsGHF45) from Monochamus saltuarius Expressed in Yeast

摘要

In this study, the cellulase of Monochamus saltuarius (MsGHF45) gene was introduced in Kluyveromyces lactis, successfully. The molecular weight of recombinant enzyme was determined by SDS-PAGE and western blotting. The enzymatic activity was confirmedby native-PAGE containing carboxymethyl cellulose as a substrate. The optimul pH and temperature of recombinant MsGHF45 was pH5 and 40°C. The barium (Ba~(2+)) and ferrous (Fe~(2+)) enhanced enzyme activity, and the mercuty (Hg~(2+)) inhibited its activity.