EFFECT OF DIFFERENT SUCROSE AND NITROGEN LEVELS IN THE MEDIUM ON CHLOROPHYLL AND ANTHOCYANIN CONTENT IN Clematis pitcheri SHOOTS CULTURED IN VITRO AT DIFFERENT TEMPERATURES

摘要

The aim of the work presented was to determine the chlorophyll and anthocyanin accumulation in Clematis pitcheri shoots cultured in vitro at different temperatures on the medium with various sucrose and nitrogen level. Two concentrations of sucrose: 10 g.l(-1) and 30 g.l(-1), and two levels of nitrogen compounds: 100% and 50% of standard MS strength, were used. Shoots were cultured at 15 degrees C, 20 degrees C and 25 degrees C. It was found that plantlets of C pitcheri grown at 20 degrees C contained the highest and at 15 degrees C the lowest content of chlorophyll. The sucrose concentration in the medium had no or only a slight effect. Lower level of nitrogen compounds (50% N) stimulated accumulation of chlorophyll in shoots as compared to a normal strength (100% N), with the highest differences at 15 degrees C. High sucrose (30 g.l(-1)) and nitrogen (100% N) concentrations and low temperature (15 degrees C) significantly promoted anthocyanins accumulation. Reduction of nitrogen compounds level in the medium to 50% and lowering sucrose concentration to 10 g.l(-1) leads to decrease of anthocyanins accumulation in the shoots cultured at 15 degrees C and 20 degrees C. In the case of explants cultured at 25 degrees C, the sucrose and nitrogen concentration in the medium had no or only a slight effect on accumulation of anthocyanins in the shoots.