A new biochemical dye molecule and nuclear stain formulation in histotechnology diagnostics as an alternative to hematoxylin

摘要

In this study, a novel molecule, a nucleotide permanent marker for nuclear staining from Papaver rhoeas (common poppy), and a sustainable stain formulation is disclosed. The molecule appears to penetrate tissue layers aggressively, and specifically attaches deoxyribonucleic acid nucleotides, staining the nucleus and revealing microscopic details. Various hematoxylin nuclear stain formulations have been presented for diagnostics, in the relative absence of viable alternatives. Millions of surgical microscopic diagnostic tests (2.5-3 million slides every day) are processed with hematoxylin, throughout the world. Apparently, hematoxylin is the most commonly used nuclear stain for histopathology diagnostics in the world, although the present cost - financial and ecological - of hematoxylin protocols is substantial and not well appreciated, even by pathologists and histologists. The origin of the hematoxylin is the Logwood tree (Haematoxylum campechianum), a species indigenous to the tropical rainforests of Central and South America, which sources the worldwide demand for a nuclear stain in medicine and raw material for other industrial disciplines. In the past, hematoxylin shortages placed hematoxylin medical diagnostics practices at risk, resulting not only in higher costs to health care, but also to the environment: increasing consumption of logwood trees deteriorated local rainforest ecology, producing negative global ecologic consequences over at least a 100-year period. Herein, a sustainable nuclear stain formulation featuring a highly complex and functional molecule, which reveals the nuclear morphology properly, is presented and the proposed nuclear staining is discussed as a promising potential substitute for hematoxylin in routine microscopic diagnostics in histology and pathology.