Optimization of extraction procedure for formaldehyde assay in smoked meat products.

摘要

To establish a formaldehyde detection method, including a method of extraction, in traditional smoked meat products, steam distillation was chosen as the preparation method. The effects of distillation time, 10% phosphoric acid solutions dosage, soaking time and water coolant flow rate on the extraction amount of formaldehyde were investigated. An orthogonal design L₉ (34) was applied to optimize the extraction conditions. The following optimum extraction conditions were determined: distillation time of 50 min, 10% phosphoric acid solution dosage of 3 mL, soaking time of 2 h and water coolant flow rate of 2.2x10-5 m3/s. The recoveries of standard additions of formaldehyde (10, 20, and 30 mg/kg) were found to be 85.79%, 91.64% and 83.45%, respectively. The repeatability relative standard deviation (RSD) was 2.669%, and the reproducibility RSD was 6.536%. The detection limit was 0.4602 mg/kg with the proposed method in smoked meat products. The smoked meat products contained high formaldehyde concentrations (up to 125 mg/kg wet weight) when the proposed method was used.