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A PLEA FOR DNA BARCODING OF FORAMINIFERA

机译:有孔虫DNA编码的请愿书

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DNA barcoding is the molecular identification of species using short, standardized gene sequences. Numerous applications of DNA barcoding in taxonomy, ecology, bioconservation, and biosafety contributed to a spectacular development of this initiative administered by the Consortium for the Barcode of Life (CBOL). Reference databases that assign DNA barcodes to particular morphospecies have been developed for almost all groups of animals, plants, and fungi, as well as some groups of protists (i.e., diatoms, ciliates, amoebae). However, such a database does not exist for foraminifera, despite large numbers of DNA sequences being available. To fill this gap, we initiated the Foram Barcoding (FB) project, whose objective is to create a curated molecular database for modern foraminifera. Each species included in our database is represented by one or several specimens, from which DNA was extracted and sequenced. Species entries include photos of processed specimens, taxonomic references, and DNA barcode sequences. A fragment of the 18S rRNA gene, commonly used in foraminiferal molecular studies, was chosen as the DNA barcode. We believe that the FB project will help resolve at least some misidentification problems that plague foraminiferal taxonomy. We also foresee its further applications in such domains of foraminiferal research as diversity assessment, ecology, biogeography, and biomonitoring. However, we are aware that the impact of the FB database depends mainly on its completeness and accuracy, and thus we appeal to the community of foraminiferologists to support this project by providing material for genetic studies and by contributing their taxonomic expertise in species identification and documentation.
机译:DNA条形码是使用短而标准化的基因序列对物种进行分子鉴定。 DNA条码在分类学,生态学,生物保护和生物安全方面的大量应用,为这项由生命条码协会(CBOL)实施的计划的惊人发展做出了贡献。已经为几乎所有动物,植物和真菌群以及一些原生生物群(即硅藻,纤毛虫,变形虫)建立了将DNA条形码分配给特定形态物种的参考数据库。但是,尽管有大量的DNA序列可用,但没有有孔虫数据库。为了填补这一空白,我们启动了Foram条形码(FB)项目,其目的是为现代有孔虫创建精选的分子数据库。我们数据库中包括的每个物种都由一个或几个标本代表,从中提取DNA并进行测序。物种条目包括处理过的样品的照片,分类参考和DNA条形码序列。选择通常在有孔虫分子研究中使用的18S rRNA基因片段作为DNA条码。我们认为,FB项目将至少帮助解决困扰有孔虫分类的一些错误识别问题。我们还预见到它在有孔虫研究领域的进一步应用,例如多样性评估,生态学,生物地理学和生物监测。但是,我们知道FB数据库的影响主要取决于其完整性和准确性,因此,我们呼吁有孔虫学家社区通过提供遗传研究材料以及贡献他们在物种识别和记录方面的分类学专业知识来支持该项目。 。

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