Computerized analysis of motility, motility patterns and motility parameters of spermatozoa of carp following short-term storage of semen.

摘要

Semen from common carp (Cyprinus carpio) was diluted in 11 buffers, and stored at 2deg, 5deg or 22deg C. Sperm motility was preserved best for 24 h by the BWW and TLP buffers. In the other buffers (fish Ringer, KCl, Cytomix, Cortland, FRT, mannitol, FPS,NAS and TSM) motility was lost after storage for 2 h. The optimum pH and temperature for storage were 6-9 and 5deg respectively. The motility characteristics observed are described. Spermatozoa exhibited linear, circular or haphazard trajectories. Some parameters of motility showed a time-dependent decrease following activation of spermatozoa with water or solutions of low osmolality.