Associations between two single nucleotide polymorphisms of the adiponectin gene, its circulating concentrations and cardiometabolic risk factors in prepubertal children with and without abdominal obesity

摘要

Background: The adiponectin gene has been identified as a susceptibility locus for metabolic syndrome, diabetes and cardiovascular disease. Aim: To examine the influence of two single nucleotide polymorphisms (SNPs) of this gene (+276G > T and +45T > G) on circulating adiponectin concentrations, and to evaluate their relationship with adiposity and cardiometabolic risk factors in prepubertal children with and without abdominal obesity. Material and methods: 168 children (78M, 6-10 yr) were examined, divided into three groups based on waist circumference (WC). Auxological and biochemical parameters were measured by standard procedures. Adiponectin SNPs were genotyped using TaqMan allelic discrimination assays. Results: Adiponectin concentration correlated inversely with measures of adiposity (r(BMIz-score)=-0.211, p(BMIz-score)=0.007; r(wc)=-0.210, p(wc)=0.008; r(wc/height)=-0.215, p(wc/height)=0.006), and was significantly influenced by blood glucose, insulin and systolic blood pressure (SBP). The +276T-allele carriers had higher SBP and diastolic BP compared to GG-homozygotes (p < 0.05), and expressed higher obesity-related measures and lower adiponectin concentrations. As to the +45T > G SNP, the GG-subject had higher total cholesterol and LDL-C concentrations compared to the T-allele carriers (p < 0.05), showing worse obesity measures, higher triglyceride, glucose and insulin and lower serum adiponectin values. Conclusion: Genetic variants of the adiponectin gene had an impact on adiposity, adiponectin concentrations and some cardiometabolic variables among prepubertal children.