Optimization of eicosapentaenoic acid production by Phaeodactylumtricornutum in the flat panel airlift (FPA) reactor

摘要

Biomass and eicosapentaenoic acid (EPA) productivities were investigated in a flat panel airlift loop reactor ideally mixed by static mixers. Growth with ammonium, urea and nitrate as nitrogen source were performed at different aeration rates. Cultures grew on ammonium but the decay of pH strongly inhibited biomass increase. On urea biomass productivity reached 2.35 g L super(-1) d super(-1) at an aeration rate of 0.66 vvm (24 h light per day, 1000 mu mol photon m super(-2) s super(-1)). Aeration rates between 0.33 vvm and 0.66 vvm and maximal productivities on urea were linearly dependent. Productivity on nitrate never exceeded 1.37 g L super(-1) d super(-1). In the range of maximum productivity photosynthesis efficiency of 10.6% was reached at low irradiance (250 mu mol photon m super(-2) s super(-1)). Photosynthesis efficiency decreased to 4.8% at 1000 mu mol photon m super(-2) s super(-1). At these high irradiances the flat panel airlift reactor showed a 35% higher volume productivity than the bubble column. At continuous culture conditions the influence of CO sub(2) concentration in the supply air was tested. Highest productivities were reached at 1.25% (v/v) CO sub(2) where the continuous culture yielded 1.04 g L super(-1) d super(-1) (16 h light per day, 1000 mu mol photon m super(-2) s super(-1)). The average EPA content amounted to 5.0% of cell dry weight, that resulted in EPA productivities of 52 mg L super(-1) d super(-1) (continuous culture, 16 h light per day) or 118 mg L super(-1) d super(-1) (batch culture, 24 h light per day).