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Virulence traits associated with verocytotoxigenic Escherichia coli O157 recovered from freshwater biofilms

机译:从淡水生物膜回收的与毒理毒素大肠杆菌O157相关的毒力特征

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Aim: To investigate whether epilithic biofilms in freshwater streams in a mixed UK agricultural river catchment harbour Escherichia coli O157, and if so, whether they demonstrate an association with those excreted by grazing farm animals. Methods and Results: Flint shingle, native to the study site, was used as a surface for biofilm development within cages of metal lath set into a stream bed at four locations on a chalkland farm. Shingle was collected from all sites once a month, as were pooled faecal samples from five farm animal populations. Subpopulations of E. coli, including E. coli O157 that demonstrated significant phenotypic and genotypic similarity with animal faecal isolates (t-test, P = 0.05) were isolated. Of 1002 E. coli isolates from biofilms and animal faeces, 48 were confirmed as the O157 strain by latex agglutination. The presence of five virulence traits associated with incidence of human disease was tested using PCR. Stx(2) was the most frequently isolated single gene (30 isolates), while stx(1) was the least frequently recovered (four isolates). Conclusion: Escherichia coli O157, expressing up to four virulence factors associated with human disease, reside within freshwater biofilms in this agricultural environment. Significance and Impact of the Study: Aquatic biofilms may potentially act as a reservoir for these pathogens, and the implications of the findings for the protection of drinking water resources should be further investigated.
机译:目的:调查英国农业河流集水区的混合大肠杆菌E157中是否存在淡水流中的上石器生物膜,如果是,则表明它们是否与放牧牲畜排出的生物膜有关。方法和结果:本研究地点原生的火石木瓦被用作生物板的表面,该生物板在白垩农场的四个位置的流化床中放置的金属板条笼内。每月从所有地点收集一次碎石,以及从五个农场动物种群中收集的粪便样本。分离出包括与动物粪便分离物具有明显的表型和基因型相似性的大肠杆菌亚群,包括大肠杆菌O157(t检验,P = 0.05)。通过生物膜和动物粪便分离出的1002株大肠杆菌中,有48株经乳胶凝集确认为O157菌株。使用PCR检测了与人类疾病发生率相关的五个毒力特征。 Stx(2)是最常分离的单个基因(30个分离株),而stx(1)是最不常分离的基因(四个分离株)。结论:在这种农业环境中,表达最多四种与人类疾病相关的毒力因子的大肠杆菌O157驻留在淡水生物膜中。研究的意义和影响:水生生物膜可能会充当这些病原体的储存库,因此,应进一步调查研究结果对保护饮用水资源的影响。

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