New carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field

摘要

Aims: Soils are rich, diversified environments where beta-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new beta-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. Methods and Results: Both libraries were screened on esculin-supplemented medium so as to isolate candidates showing beta-glucosidase activity. Candidate analysis revealed seven putative beta-glycosidases and two putative glycosyltransferases, displaying 25 to 82% identity to known enzymes. The putative beta-glycosidases belong to families GH1, GH3 and GH20 and the two putative glycosyltransferases, probably, to new families. In characterization tests performed on bacteria in suspension or spread on agar plates, some candidates appeared to hydrolyse several natural and synthetic substrates. These tests also highlighted interesting industrial characteristics, such as the activity of four beta-glycosidases under alkaline conditions and the esculin-hydrolysing activity of a beta-glucosidase candidate in the presence of glucose. Conclusions: Seven putative beta-glycosidases and two putative glycosyltransferases were found by functional screening of two metagenomic DNA libraries derived from agricultural soil. Significance and Impact of the Study: This study has identified beta-glycosidases and putative glycosyltransferases that have or may have interesting industrial characteristics