IDENTIFICATION AND CHARACTERIZATION OF LACTOBACILLUS SPECIES ISOLATED FROM FILLETS OF VACUUM-PACKED SMOKED AND SALTED HERRING (CLUPEA HARENGUS)

摘要

Seventy-eight strains were isolated from fillets of vacuum-packed smoked and salted herring (Clupea harengus) on APT glucose medium at 5 degrees C or 20 degrees C. All the isolates belonged to the genus Lactobacillus. CO2 production and arginine degradation were characteristic of the isolates, A dichotomous key is proposed, using only four characters (growth on Rogosa broth, fermentation of ribose, presence of meso-diaminopimelic acid, isomer of lactate produced) for presumptive identification of lactobacilli from fish, The isolates could be allocated to three groups related to Lactobacillus groups II or III of Kandler and Weiss. Most of the strains possess important technological capacities: tolerance of high levels of NaCl, liquid smoke and bile salts. No lipolytic activity was found but some proteolytic activity was found on casein. Inhibitor activity against pathogenic or spoilage organisms was frequent and probably due to the production of hydrogen peroxide, or organic acids or both. No Carnobacterium strains were isolated despite the use of suitable temperature and isolation medium, probably because of the smoking of the fish.