Procedure for the quantification of the biomarker (2-methoxyethoxy) acetic acid in human urine samples

摘要

An accurate and precise procedure was developed for the detection and quantification of (2-methoxyethoxy) acetic acid (MEAA), a metabolite and biomarker for human exposure to 2-(2-methoxyethoxy) ethanol. The compound 2-(2-methoxyethoxy) ethanol has a wide array of industrial applications including its use as an additive in military jet fuel. Exposure to 2-(2-methoxyethoxy) ethanol is a health concern owing to its toxicity which includes developmental and teratogenic properties. Sample preparation consisted of liquid-liquid extraction (LLE) and esterification of MEAA to produce the ethyl ester. Measurement was by a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a HP-1 capillary column. Recovery studies of spiked blank urine demonstrated good accuracy and precision; recovery varied between 95 and 103% with relative standard deviations of 8.6% and less. The limit of detection (LOD) for this procedure was found to range from 0.02 to 0.08 μg/ml equivalent levels of MEAA in urine. These data and other aspects of the Published by Elsevier B.V.