Large-volume stacking for quantitative analysis of anions in capillary electrophoresis II. Large-volume stacking without polarity switching

摘要

Several methods have been reported for performing on-column concentration for sensitivity enhancement in high-performance capillary electrophoresis One technique of interest consists of a large-volume hydrodynamic injection, followed by the removal ofthe large plug of low conductivity sample matrix from the capillary. In this work, this removal is performed using the redissolution of an electroosmotic flow modifier that will act as a pump, without the need for polarity switching, The performance ofthis method is evaluated and optimized for the separation of arsenious acids. Then, the possibility of the quantitative aspect of this stacking procedure is discussed.