TIME-RESOLVED PHOTON-COUNTING FLUORIMETRY WITH A SEMICONDUCTOR LASER FOR CAPILLARY ELECTROPHORESIS

摘要

Effects of the gate width and the gate delay on the signal-to-noise ratio were investigated for a laser-induced fluorescence detector of capillary electrophoresis using a pulsed semiconductor laser, a photomultiplier and a time-resolved photon counting method. Separation of fluorescence and background was easier for a fluoropher with a long lifetime.