ANALYSIS OF THE 9-FLUORENYLMETHOXYCARBONYL HYDRAZIDE LABELLING OF NEUTRAL AND SIALIC ACID-CONTAINING OLIGOSACCHARIDES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

Maltoheptaose (M7) and neuraminyllactose (NeuAc-Lac) as models of neutral and sialyl oligosaccharides were derivatized with 9-fluorenylmethoxycarbonyl hydrazide (FMOC hydrazide) at 37 degrees C in acetonitrile-water (50:50) containing 0.5-5% acetic acid without causing any desialylation. The reproducibility of the derivatization of M7 with FMOC-hydrazide was examined and the average relative standard deviation (n=9) was 2.8%. The oligosaccharide FMOC hydrazones were separated on a Cosmosil C-18 column by isocractic elution. The determination limits of M7 and NeuAc-Lac derivatives are at the picomole level for ultraviolet detection and the sub-picomole level for fluorescence detection.