Hydrophilic interaction liquid chromatography-mass spectrometric imaging platform for N-glycan relative quantitation using stable-isotope labeled hydrazide reagents

摘要

Various factors including labeling reagent impurity, labeling efficiency difference, stability of the labeled sample, and different labelling ratio were explored to ensure accurate quantitation was achieved in MALDI mode. The feasibility and reliability of HILIC-MALDI MSI platform for reliable quantitative analysis of N-glycans was demonstrated using heavy and light labeled maltodextrin at 1:1 ratio.