HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHIC CHARACTERIZATION OF WATER-SOLUBLE POLYMERIC SUBSTANCES PRODUCED BY PHANEROCHAETE CHRYSOSPORIUM FROM FREE AND WHEAT CELL WALL BOUND 3,4-DICHLOROANILINE

摘要

A high-performance size-exclusion chromatographic (HPSEC) method was developed and optimized for the separation of aq, soluble polymeric xenobiotics. The use of this method for the characterization of aq. soluble polymeric substances produced by Phanerochaete chrysosporium cultures from [ring-(UC)-C-14]-3,4-dichloroaniline ([C-14]DCA) and wheat cell wall bound [ring-U-C-14]-3,4-dichloroaniline was demonstrated by on-line UV detection at 280 nm and radioactivity tracing. The polymeric nature of aq, soluble lignin-like C-14-products was shown by polydispersity and correspondence of the UV signal at 280 nm with the radioactivity trace. The higher polydispersity and molecular mass of lignin-like substances in low-C cultures as compared to those in low-N cultures were found to be associated with lower mineralization rates. Results indicated that the major portion of copolymerized DCA was apparently covalently bound via labile bonds to the alpha-C atom of the lignin monomer side chains on native wheat cell wall. [References: 18]