MINIMIZATION OF BASELINE DISTURBANCES IN PEPTIDE MAPPING BY MEANS OF PRINCIPAL-COMPONENT MULTIVARIATE VISUALIZATION

摘要

Optimum method parameters for minimizing baseline disturbances in gradient HPLC peptide mapping were identified by the use of principal component mapping. Gradient baseline disturbances were attributed to differences in extinction coefficients between low concentrations of trifluoroacetic acid (0.1%, v/v) in water and in acetonitrile mobile phases. Method parameters that were investigated included (1) column differences, (2) trifluoroacetic acid concentration, (3) mobile phase mixer configuration and (4) detector wavelength. Items (1) and (3) were identified as the most significant method parameters. [References: 5]