Determination of the Enantiomeric Composition of Limonene and Limonene-l,2-epoxide in Lemon Peel by Multidimensional Gas Chromatography with Flame-Ionization Detection and Selected Ion Monitoring Mass Spectrometry

摘要

The enantiomeric excess of limonene and limonene-l,2-epoxide in lemon peel samples is determined. The enantiomers were separated by multidimensional gas chromatography with flame ionization detection and gas chromatography-mass spectrometry in the selected ion monitoring mode. With this system, it is possible to measure limonene-l,2-epoxide, which appears at low concentrations in lemon peel. The enantiomeric excess of R-(+)-limonene in lemon peel is between 97.1 and 97.4% and that of (1S,2R,4R)-(+)-limonene-l,2-epoxide is between 88.0 and 91.9%. The quantitative determination of limonene in lemon peel is carried out using the optical antipode of the limonene to be quantitated as an internal standard.