Screening Method for M-Proteins in Serum Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry

Kohlhagen Mindy C.; Barnidge David R.; Mills John R.; Stoner Joshua; Gurtner Kari M.; Liptac Andrea M.; Lofgren Denise I.; Vanderboom Patrick M.; Dispenzieri Angela; Katzmann Jerry A.; Willrich Maria A. V.; Snyder Melissa R.; Murray David L.

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Screening Method for M-Proteins in Serum Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry

机译：纳米抗体富集-MALDI-TOF质谱法筛选血清中M蛋白的方法

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BACKGROUND: Current recommendations for screening for monoclonal gammopathies include serum protein electrophoresis (PEL), imunofixation electrophoresis (IFE), and free light chain (FLC) ratios to identify or rule out an M-protein. The aim of this study was to examine the feasibility of an assay based on immunoenrichment and MALDI-TOF-MS (MASS-SCREEN) to qualitatively screen for M-proteins.

机译：背景：目前筛查单克隆血友病的建议包括血清蛋白电泳（PEL），免疫固定电泳（IFE）和自由轻链（FLC）比率，以鉴定或排除M蛋白。这项研究的目的是检验基于免疫富集和MALDI-TOF-MS（MASS-SCREEN）进行定性筛选M蛋白的方法的可行性。

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《Clinical Chemistry: Journal of the American Association for Clinical Chemists》 |2016年第10期|共8页
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Kohlhagen Mindy C.; Barnidge David R.; Mills John R.; Stoner Joshua; Gurtner Kari M.; Liptac Andrea M.; Lofgren Denise I.; Vanderboom Patrick M.; Dispenzieri Angela; Katzmann Jerry A.; Willrich Maria A. V.; Snyder Melissa R.; Murray David L.;
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正文语种 eng
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1. Screening Method for M-Proteins in Serum Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry [J] . Kohlhagen Mindy C., Barnidge David R., Mills John R., Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2016,第10期

机译：纳米抗体富集-MALDI-TOF质谱法筛选血清中M蛋白的方法
2. Comprehensive Assessment of M-Proteins Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry [J] . Angela Dispenzieri, David L. Murray, David R. Barnidge, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2016,第10期

机译：纳米抗体富集与MALDI-TOF质谱联用对M蛋白进行综合评估
3. Investigating redox regulation of protein tyrosine phosphatases using low pH thiol labeling and enrichment strategies coupled to MALDI-TOF mass spectrometry [J] . BonhamC.A., SteevenszA.J., GengQ., Methods: A Companion to Methods in Enzymology . 2014,第2期

机译：使用低pH巯基标记和富集策略结合MALDI-TOF质谱研究蛋白质酪氨酸磷酸酶的氧化还原调节
4. Liquid Chromatography coupled to High Resolution Mass Spectrometry methods for the global metabolic profiling of human serum [C] . Samia Boudah, Marie-Francoise Olivier, Sandrine Aros-Calt, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：液相色谱耦合到高分辨率质谱法，用于全球血清的全球代谢谱
5. Selective Drug Screening Method Development using Nanofibers and Microfluidics Coupled to a Quadrupole Time-of-Flight Mass Spectrometry [D] . Chen, Yufei. 2018

机译：使用纳米纤维和微流体的选择性药物筛选方法开发，耦合到四极飞行时间质谱法
6. Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry [O] . Leshan Xiu, Chi Zhang, Zhiqiang Wu, -1

机译：MALDI-TOF质谱法通用冠状病毒筛选方法的建立与应用
7. Comprehensive Assessment of M-Proteins Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry [O] . John R Mills, Mindy C Kohlhagen, Surendra Dasari, 2016

机译：使用纳米抗体富集的M-蛋白综合评估偶联至MALDI-TOF质谱法

Screening Method for M-Proteins in Serum Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry

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