Alkaline phosphatase immobilization onto Bio-Gide? and Bio-Oss? for periodontal and bone regeneration

摘要

Aim To evaluate the effect of alkaline phosphatase (ALP) immobilization onto Bio-Gide? in vitro, and to study the in vivo performance of ALP-enriched Bio-Gide? and/or Bio-Oss? with the purpose to enhance periodontal regeneration. Materials and Methods Alkaline phosphatase ALP was immobilized onto Bio-Gide? and Bio-Oss?. Forty-eight rats received periodontal defects, which were treated according to one of the following strategies: Bio-Gide?, Bio-Gide?-ALP, Bio-Gide?-ALP/Bio-Oss?, Bio-Gide?/Bio-Oss?-ALP, Bio-Gide?-ALP/Bio-Oss?-ALP, or empty. Micro-CT and histological analysis were performed. Results A 30 min ALP-deposition time was determined as optimal from mineralization capacity assessment and consequently used as Bio-Gide?-ALP membranes in the animal experiment. In vivo results showed that after 2 weeks, the defect and implanted materials were still visible, an inflammatory response was present, and membrane degradation was ongoing. Bone formation, although limited, was observed in the majority of Bio-Gide?-ALP specimens and all of the Bio-Gide?/Bio- Oss?-ALP specimens, and was significantly higher compared with Bio-Gide? and empty controls. After 6 weeks, the defects and particles were still visible, whereas membranes were completely degraded. The inflammatory response was decreased and bone formation appeared superior for Bio-Gide?-ALP treated defects. Conclusion Immobilization of ALP onto guided tissue regeneration (GTR)/ guided bone regeneration (GBR)-materials (Bio-Gide? and Bio-Oss?) can enhance the performance of these materials in GTR/GBR procedures.